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KMID : 1140120110160010001
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Cancer Prevention Research
2011 Volume.16 No. 1 p.1 ~ p.12
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Application of DNA Repair Analysis Using Comet Assay in Cancer Prevention
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Lee Hyun-Jung
Park Eun-Ju
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Abstract
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DNA repair plays important roles in maintaining a low steady state level of DNA damage and protecting us from genetic mutation and cancer development. The comet assay, a fast, simple, and sensitive technique for the analysis of DNA damage and repair on cells, has applications in testing novel chemicals for genotoxicity, monitoring environmental contamination with genotoxins, and population monitoring. Alkaline comet assay is a method used to measure single strand breaks and alkali-site. The sensitivity and specificity of the comet assay are greatly enhanced if the nucleoids are incubated with lesion-specific enzymes that recognize specific lesion in the DNA. In addition, the repair activity in a cell extract can be measured by incubating it with nucleoids containing specific damage. The comet assay can be modified to measure DNA incision activity reflecting base excision repair and nucleotide excision repair. Currently, only a few studies have investigated the influence of nutritional factor diseases such as cancer, metabolic disease on DNA repair. In this review we addressed the application of comet assay to detect DNA repair capacity, focusing on the methodology and recent research trends.
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KEYWORD
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DNA repair, Comet assay, Cancer prevention
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